Table 1 Edits of the FGF20 gene based on ssODN donor used for allelic repair in single-cell clones targeted with Sca-ssODN and SpCas9 or SpCas9-HF1 (Supplementary FigsĀ S4 and S5).

From: High fidelity CRISPR/Cas9 increases precise monoallelic and biallelic editing events in primordial germ cells

Clone genotype

Sca/Sca

Sca/Sca*Wt

Sca/INDEL

INDEL/INDEL

Total

SpCas9-WT clones

0

0

3 (7.9%)

35 (92.1%)

38 (100%)

SpCas9-HF1 clones

14 (36.8%)

2 (5.3%)

7 (18.4%)

15 (39.5%)

38 (100%)

HDR

Biallelic

Biallelic

Monoallelic

N/A

N/A