Table 3 Edits of FGF20 gene based on ssODN donor used for allelic repair single-cell clones targeted with SpCas9-HF1 and a mixture of Sca-ssODN and Silent2-ssODN (Supplementary Fig. S7).

From: High fidelity CRISPR/Cas9 increases precise monoallelic and biallelic editing events in primordial germ cells

Clone genotype

Sca/Silent2

Sca/Sca

Silent2/Silent2

Silent2/Silent2*Wt

Sca/INDEL

Silent2/INDEL

INDEL/INDEL

Total

SpCas9-HF1 clones

9 (25.0%)

5 (13.9%)

8 (22.2%)

2 (5.6%)

3 (8.3%)

1 (2.8%)

8 (22.2%)

36 (100%)

HDR

Biallelic

Biallelic

Biallelic

Biallelic

Monoallelic

Monoallelic

N/A

N/A

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