Table 2 Pronuclear formation pattern of cloned embryos derived from faecal cell nucleus.
From: Generation of two-cell cloned embryos from mouse faecal cell
Donor cell type | Donor cell group | No. of cloned embryos performed immunostaining | No. of each pattern of pronuclear formation at 8 hpa (%) | |||
|---|---|---|---|---|---|---|
Pattern A | Pattern B | Pattern C | Pattern D | |||
Faeces-derived cell | GFP+, Hoechst+ | 18 | 1 (5.6) | 4 (22.2) | 13 (72.3) | 0 (0) |
|  | GFP−, Hoechst+ | 19 | 2 (10.5) | 4 (21.1) | 13 (68.4) | 0 (0) |
|  | GFP+, Hoechst− | 13 | 0 (0) | 0 (0) | 1 (7.7) | 12 (92.3) |
Cumulus cell | — | 11 | 11 (100) | 0 (0) | 0 (0) | 0 (0) |
