Table 3 Development of parthenogenetic embryos injected with faecal cell nucleus or suspension.
From: Generation of two-cell cloned embryos from mouse faecal cell
| Â | Injection material | No. of oocytes | No. of PN formation at 8 hpa (%) | No. of embryos at 24 hpa (%) | No. of embryos at 48 hpa (%) | No. of embryos at 72 hpa (%) | No. of embryos at 96 hpa (%) | |||
|---|---|---|---|---|---|---|---|---|---|---|
2-cell | 1-cell | Fragmented | Died | 4–8 cell | Morulae/ Blastocyst | Blastocyst | ||||
Parthenogenesis after injection into MII oocyte | faecal cell (Hoechst+) | 70 | 68 (97.1) | 7 (10.3) | 39 (57.4) | 21 (30.9) | 3 (4.4) | 2 (2.9) | 0 (0) | 0 (0) |
faecal suspension | 44 | 43 (97.7) | 8 (18.6) | 33 (76.7) | 3 (7.0) | 0 (0) | 3 (7.0) | 3 (7.0) | 1 (2.3) | |
PBS | 45 | 43 (95.6) | 40 (93.0) | 3 (7.0) | 2 (4.7) | 0 (0) | 39 (90.7) | 38 (88.4) | 33 (76.7) | |
Parthenogenesis (control) | none | 40 | 35 (87.5) | 35 (100) | 0 (0) | 0 (0) | 0 (0) | 35 (100) | 34 (97.1) | 33 (94.3) |
