Figure 1
From: Engineering a branching sucrase for flavonoid glucoside diversification

View of the α-(1→2) branching sucrase active site pocket showing the 4 amino acid mutagenesis targets of each library (W2135 and F2136 in light green and F2163 and L2166 in magenta) and the adjacent ones (colored respectively in blue and light pink). Quercetin mono-glucosides (A) glucosylation at C3′ and C4′ of ring A; B: glucosylation at C5 and C7 of ring A) are docked in the active site and represented in sticks. The nucleophile (D2210) and the acid/base (E2248) catalysts are shown in black for reference.