Table 1 Main features of the 22 selected α-(1→2) branching sucrase mutants.
From: Engineering a branching sucrase for flavonoid glucoside diversification
Name | AA at position | Relative activity on sucrose | Quercetin conversion rate (%) | |||
|---|---|---|---|---|---|---|
2135 | 2136 | 2163 | 2166 | |||
WT | W | F | F | L | 1.0 ± 0.2a | 62 ± 7a |
A10 | V | F | F | L | 2.0 | 97 |
B5 | C | I | F | L | 0.3 | 99 |
C12 | S | L | F | L | 0.8 | 97 |
C1 | I | C | F | L | 0.5 | 97 |
C2 | N | Y | F | L | 0.4 | 91 |
C8 | N | F | F | L | 0.3 | 91 |
D10 | I | Y | F | L | 1.7 | 75 |
E2 | C | F | F | L | 0.5 | 99 |
E12 | L | F | F | L | 0.4 | 98 |
F3 | N | H | F | L | 0.3 | 91 |
F4 | L | L | F | L | 0.4 | 97 |
G1 | F | I | F | L | 0.5 | 98 |
H1 | C | N | F | L | 0.5 | 97 |
H5 | F | F | F | L | 2.0 | 91 |
H10 | G | F | F | L | 0.4 | 98 |
B’2 | W | F | G | L | 0.5 | 78 |
B’5 | W | F | F | I | 0.3 | 68 |
C’5 | W | F | H | L | 0.4 | 76 |
E’10 | W | F | G | I | 0.4 | 65 |
F’9b | W | F | L | L | 0.7 | 89 |
G’5 | W | F | L | L | 0.9 | 87 |
H’5c | W | F | I | I | 1.0 | 70 |
