Figure 6
Overexpression of miR-494-3p reduces oxygen consumption rate and Ucp1 expression in primary adipocytes. (A,B) Oxygen consumption rate (OCR) in primary beige adipocytes with miR-494-3p overexpression (A) or inhibition (B) treated with isoproterenol (50 µM), oligomycin (2 µM), FCCP (2 µM), rotenone (R; 0.5 µM) and antimycin A (AA; 0.5 µM) as indicated. n = 5. (C,D) Representative images of UCP1 immunofluorescence staining (green) and Mitotracker red staining (red) in primary differentiated beige adipocytes with miR-494-3p overexpression (C) or inhibition (D). Cells were harvested at day 8 with iso (10 µM) treatment for 8 h. (E,F) Quantification of the merge area (yellow) of GFP-mito (green) and mito-red (red) and number of adipocytes (indicated by DAPI) using ImageJ in cells with miR-494-3p overexpression (E) or inhibition (F). n = 8–10. *p < 0.05, **p < 0.01. Ucp1: uncoupling protein 1; iso: isoproterenol; Oligo: oligomycin; RAA: rotenone and antimycin A.
