Figure 3 | Scientific Reports

Figure 3

From: Characterization of a novel AICARFT inhibitor which potently elevates ZMP and has anti-tumor activity in murine models

Figure 3

(A) The AMPK T172 and P70S6K T389 phosphorylation levels after overnight treatment with 0 to 20 μM LSN3213128 in (A) NCI-H460, (C) MDA-MB-231met2, and (E) A9 using RPMI media, i.e. regular folate conditions. The levels of total actin, AMPKα and P70S6K are also shown. The anti-proliferative effect of LSN3213128 with (blue) and without (red) hypoxanthine in tissue culture are shown as measured using Alamar Blue in (B) NCI-H460, (D) MDA-MB-231met2, and (F) A9. Full images and the croppings used in this figure are shown in Supplemental Figures S4–S13.

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