Table 1 Bat specimens included in this study and confirmation of absence of ORF8.

From: Attenuation of replication by a 29 nucleotide deletion in SARS-coronavirus acquired during the early stages of human-to-human transmission

Country

N individuals tested

Rhinolophid species and number of individuals (n) yielding positive RT-PCR

Number of RT-PCR results indicating absence of ORF8

Bulgaria

506

R. euryale (n = 44)

R. blasii (n = 11)

R. ferrumequinum (n = 1)

R. mehelyi (n = 4)

44

11

1

4

Italy

45

R. ferrumequinum (n = 6)

6

Slovenia53

36

R. hipposideros (n = 5)

5

Spain

285

R. hipposideros (n = 21)

21

Total

872

All 5 species known in Europe

92 (100%)

  1. Absence of ORF8 was determined by RT-PCR using reverse primers F29260R (TTTGTATGCGTCAATGTGCTTG) for reverse transcription and F28182R (GGGTCCACCAAATGTAATGCGG) and forward primer F27626F (GAGAAAGACAGAATGAATGAGC) for PCR.
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