Figure 2

Sequential gating to identify AdPCs in panel 1. (A) Delimitation of cell gate in the FSD-SSC plot, (B) Identification of CD45− cells. (C) Identification of AdPCs (CD45−/CD34+/CD31−) and endothelial cells (CD45−/CD31+). Events negative for CD34 and CD31 come majorly from tissue debris. A, B and C belong to the same sample (visceral adipose tissue of a morbid obese patient). (D) Identification of AdPCs and endothelial cells from another sample (subcutaneous adipose tissue of a morbid obese patient).