Figure 4 | Scientific Reports

Figure 4

From: Age-related waning of immune responses to BCG in healthy children supports the need for a booster dose of BCG in TB endemic countries

Figure 4

Regulatory T cells (A) Representative plots from a healthy control child are shown. From left to right, CD3 vs Time is shown to detect differences in flow. Cell doublets were excluded with forward scatter area versus forward scatter height parameters. Plotting FSC-A vs SSC-A allows identification of the lymphocyte population based on size and granularity. CD4+CD3+T cells were identified, followed by CD4+CD25+T cells. Using FMO gates, FOXP3 and CD39 gates were placed to identify the CD3+CD4+CD25+CD39+FOXP3+ population. Age-related frequency of regulatory T cells in (B) unstimulated whole blood and (C) whole blood incubated with BCG for 20 hours. Horizontal bars represent median values.

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