Figure 1

Schematic fragment ion intensity chromatogram of Rutin (Ru), its quantification in AM-HM and plasma using LC-MS/MS. Chromatogram of Ru present in hydro-alcoholic extract of leaves of Aegle marmelos (AM-HM,1 mg/ml) and standard Ru used for MS2 fragmentation (a,b). Representative chromatogram of blank human plasma wherein no peak of Ru could be detected (c). Well separated, finely resolved, sharp peak of Ru could be detected at 1 h time point (d) in plasma following administration of AM-HM (500 mg/kg, po) to normal rat.