Figure 6

Effect of AM-HM on the milieu of sugar metabolizing enzymes of HepG2 when they are grown in fructose-rich media. Treatment of HepG2 cells grown in media either without glucose (NC) or with 0.55 mM fructose(FC1) or with 1 mM fructose (FC2) or with 1 mM fructose + 0.1 µM Insulin (FC3) with AM-HM (75 µg/ml), AM-H (75 µg/ml), AM-C (75 µg/ml), AM-EA (75 µg/ml), AM-B (75 µg/ml) or AM-A (75 µg/ml) for 48 hours affected the glycogen content (µg/ml), activities of glycolytic enzymes-hexokinase (IU/ml), aldehyde dehydrogenase(IU/ml), ketohexokinase (ng/ml) and irreversible regulator of glycolysis-phosphofructokinase (ng/ml). The AM-HM and AM-B reduced the glycogen level in FC1 & FC2 and the effect was augmented by insulin in FC3. The AM-HM and AM-B potentiated the action of HK in FC1 & FC2 and the effect was augmented by insulin in FC3. The AM-HM and AM-B reduced the activities of ALDH, KHK and PFK in FC1 & FC2 and the effect was augmented by insulin in FC3. Each value is the mean ± SD of n = 3. *p < 0.05 vs control using student-t-test.