Figure 1
From: Simultaneous targeting of linked loci in mouse embryos using base editing
Simultaneously targeting of two linked genomic loci by cytosine-deaminase-mediated base editing. (a) Schematic diagram of target sites in the casein locus. The eight putative enhancers (A-H) and the two promoters in the Csn2 and Csn1s2a locus were identified by ChIP-seq analysis for enhancer marks. Sites C and E are 9 kb apart and were targeted simultaneously with two sgRNAs and VQR-BE3 and BE4. (b) Summary of data obtained from mouse zygotes co-injected with VQR-BE3 and BE4 mRNA, and two sgRNAs. Experiments were conducted with founder mice and established mouse lines. (c) C-to-T mutation frequency observed at the two sites. (d) Distribution of linked (on the same chromosome) and non-linked mutations.
