Figure 2
From: Simultaneous targeting of linked loci in mouse embryos using base editing
Alignment of sequences from founder mice carrying mutations in sites C and E. (a) sgRNA sequences are underlined and the PAM sites are shown in brown. The C-to-T on-target substitutions are shown in green. Mutant mice carrying homozygous mutations are marked in bold purple. Unintended nucleotide substitutions are shown in red. Deletions are shown as underlines. WT, wild-type. (b) Sanger sequencing chromatograms of DNA from WT and mutant mice carrying homozygous mutations (founder 166, 169, 175, and 186). The sgRNA sequences are underlined. C-to-T transitions are seen at target sites C and E and marked with a red asterisk.
