Figure 4
From: Simultaneous targeting of linked loci in mouse embryos using base editing
Biological consequence of enhancer mutations during pregnancy. (a–c) Expression of the Csn2 and Csn1s2a genes in mammary tissue from WT and mutant mice carrying nucleotide substitution at site C or E, respectively, by qRT-PCR. mRNA levels were normalized to Gapdh levels. Results are shown as the means ± s.e.m. of independent biological replicates (WT and mutants, n = 3). T-test was used to evaluate the statistical significance of differences between WT and mutants. ****P < 0.00001. n.s., not significant.
