Figure 2

AO staining for autophagosome formation in ARPE-19 cells after cells were treated with escalating concentrations of H2O2 for 24 hours. (a) Untreated cells, (b) Cells treated with 100 μM H2O2. (c) Cells treated with 200 μM H2O2. (d) Cells treated with 300 μM H2O2. (e) Cells treated with 400 μM H2O2. Each group of experiments was repeated 3 times and a representative photo is shown. The images were photographed using 10× objects.