Figure 4

STS decreases autophagic protein expression in ARPE-19 cells under oxidative stress. (a) Representative protein gel blots of Beclin 1, ATG3, ATG7 and ATG9 are shown. Actin was utilized as a loading control. (b–e) The accompanied histogram of densitometric quantification of Beclin1, ATG3, ATG7 and ATG9. Untreated cells were used as positive control. Experiments in each group were repeated 3 times. Data were analyzed using one- way ANOVA, and differences between means were considered statistically significant when P < 0.05, Bonferroni correction of P-value was applied in multiple comparison. Statistical analysis showed that there was no significant difference between H₂O₂ + STS and H₂O₂ + 3MA in C and E, and the other groups showed statistically significant differences.