Figure 3

AGO2 and PUM proteins show overlap at the binding site level. (A) Density of PUM1 (light blue) and PUM2 (dark blue) binding sites surrounding AGO2 binding sites. The shaded area represents a mean +/− 1 SD interval of 100 control densities where PUM1 site locations were randomized within each 3′UTR, and distances to AGO2 peaks computed. (B) Density of Targetscan7 predictions surrounding AGO2 peaks, separated by sites that overlap with PUM1 (light blue), PUM2 (dark blue), both (black), or neither (red). The shaded area represents randomized PUM1-only controls as above. (C,D) Density of PUM motifs surrounding PUM1 (C) and PUM2 (D) sites, separated by populations that do (red) or do not (blue) overlap with AGO2. The shaded areas represent randomized controls for the corresponding populations.