Figure 4
From: Antagonistic and cooperative AGO2-PUM interactions in regulating mRNAs
AGO2-PUM site co-occupancy affects PUM binding. (A) Schematic of possible interactions between AGO2 and the PUM proteins for PUM sites with and without AGO2 overlap, as well as predicted effect of DICER knockdown in each case. (B) Cumulative distribution plots of the log fold change in PUM1 site strength, with (red) and without (light blue) AGO2 overlap. (C) Cumulative distribution plots of the log fold change in PUM2 site strength, with (red) and without (dark blue) AGO2 overlap. (D) Example UCSC browser view of a site with overlapping PUM1 (light blue), PUM2 (dark blue), and AGO2 (red) binding within the RRAGD 3′UTR. PUM1 and PUM2 binding sites show overlap with an independently generated PUM2 PAR-CLIP and are in close proximity to a PUM motif. The AGO2 binding site contains a predicted miR-17 site.
