Figure 6
From: Antagonistic and cooperative AGO2-PUM interactions in regulating mRNAs
PUM antagonizes AGO through the predicted PUM motif, and PUM double knockout stabilizes endogenous transcripts. (A) Sites co-regulated by AGO and PUM show dependencies on specific miRNAs, PUM proteins, and the predicted PUM site. Luciferase reporter constructs co-transfected with control or specific miRNA inhibitors were tested under wildtype (white) and PUM double knockout (black) conditions with WT (solid) or mutant PUM sites (stripes). (B) RT-qPCR of endogenous VLDLR and RRAGD transcripts in both wildtype (white) and PUM double knockout (black) cells. (C) A model of the antagonistic effect of PUM on AGO2 regulation of VLDLR and RRAGD mRNAs.
