Figure 3
Activated top upstream regulators in mice infected with N67 and 17XNL P. yoelii parasites. Genes differentially expressed in N67- or 17XNL-infected and uninfected mice with a cutoff value of 2.0-fold or larger changes were selected for analysis using Ingenuity Pathway Analysis (IPA). (a) Signaling pathways derived from analysis of top upstream regulators after infection with N67 and/or 17XNL. TLR and cytosolic sensor mediated signaling pathways leading to production of IFN-I and pro-inflammatory cytokines (TNF and IFN-γ) are detected (or predicted such as LPS). Molecules shown are selected upstream regulators significantly activated (p < 0.001 and z-score ≥ 3.0) in mice day 1 post infection with N67 parasite as predicted by IPA analysis (also see Supplementary Table S5). (b) Western blot of protein expression and/or phosphorylation day 1 and day 4 after infection with N67 or 17XNL. Proteins from spleen tissues of infected mice were separated on SDS-PAGE gels, transferred to PVDF membrane, and probed with protein or phosphorylation specific antibodies after blotting to NC membrane. The full gel images for these Western blots are presented in Supplementary Fig. 6.
