Figure 3
Response of the YTnC indicator to Ca2+ variations in HeLa Kyoto cells and cultured neurons. (a) Confocal images of HeLa Kyoto cells co-expressing green YTnC (panel a, left) and red R-GECO1 (panel a, right) calcium indicators. (b, c) The graphs illustrate changes in green fluorescence of YTnC (panel b) or control NTnC (panel c) indicators and in red fluorescence of the reference co-expressed R-GECO1 GECI in response to the addition of 2 mM CaCl2 and 2.5 μM ionomycin. The changes in panel b correspond to the area indicated with white circles in panel a. One example of three repeats is shown. (d) Dissociated neuronal culture co-expressing YTnC (panel d, left) and R-GECO1 (panel d, right) calcium indicators. (e,f) The graphs illustrate changes in red fluorescence of R-GECO1 (excitation 561 nm) and green fluorescence of YTnC (excitation 488 nm) (panel e) or control NTnC (panel f) as a result of spontaneous activity in neuronal culture. The graph in panel e illustrates changes in fluorescence in the area indicated with white circle in panel d. (b–c, e–f) The minimal fluorescence values were normalized to one.
