Figure 5
In vivo drifting grating-evoked neuronal activity in the mouse cortex visualized with YTnC and GCaMP6s calcium indicators using two-photon microscopy. (a) Example of the 3D reconstruction of YTnC-positive cells in the V1 visual cortex area using 960 nm excitation light. Block size, 317 × 317 × 350 μm. (b) Averaged ΔF/F responses corresponding to spontaneous (non-specific) and grating-evoked (specific) activity across neurons (n = 9, YTnC; n = 5, GCaMP6s and n = 3, YTnC; n = 2, GCaMP6s, respectively) in the V1 area for the YTnC and GCaMP6s indicators, respectively. (c) Two-photon images of two regions from V1 layer 2/3 neurons captured during presentation of drifting grating to the mouse for YTnC and GCaMP6s indicators. Averaged ΔF/F responses during presentation of drifting gratings (eight directions, 10 repetitions) are shown for the selected neurons. The directions of the drifting gratings (blue lines) are shown with arrows (in black). Grey and red lines correspond to the individual and averaged traces across ten repetitions, respectively. Black horizontal lines correspond to the time of grating presentation.
