Figure 6
In vivo neuronal Ca2+ activity in hippocampus of freely behaving mice visualized with calcium indicators YTnC, GCaMP6f, GCaMP6s and an nVista HD system. (a) Photo of an nVista HD miniature microscope mounted to the head of mice. (b) Spatial filters and sample traces obtained from a 10-min imaging session with freely behaving mice expressing GCaMP6f, YTnC and GCaMP6s. (c) Mean spikes for calcium indicators GCaMP6f, YTnC and GCaMP6s; spikes exceeding the 4 MAD threshold were aligned at the moment of the very start of the peak (3 s). (d) Photo of O-shaped track with landmarks. (e) Circular plot of mouse trajectory during the exploration of circular track, synchronized with the spikes of a place cell (green triangles). The sensors GCaMP6f, YTnC and GCaMP6s were delivered to the hippocampus with rAAV (AAV-CAG-NES-GCaMP6f, AAV-CAG-NES-YTnC and AAV-CAG-GCaMP6s, respectively) particles.
