Figure 2
PCR success rate distribution for the 136 NPCL marker candidates and mutation rates for the 63 universal NPCL markers. (A) PCR success rate distribution for 136 candidates in 23 avian orders. The 63 NPCL markers with PCR success rates higher than 80% are shown in black; other loci with PSR success rates below 80% (in grey) were excluded from the subsequent analysis. The number above each bar shows the number of NPCL marker candidates. (B) Mutation and success rates for the 63 universal NPCL markers. The markers were sorted according to estimated mutation rates (bars in black) from low to high. The number on the right of each bar is the mutation rate of each NPCL marker. PCR success rates are shown underneath (bars in grey). The mutation rates of widely-used NPCL RAG1 (in green) and mitochondrial gene cyt b (in blue) were selected as references.
