Figure 4

SHP deficiency in liver parenchymal cells promotes neutrophil infiltration to the injured liver. (a–c) Liver immune cells were isolated 3 h after challenge with 25 mg/kg of ConA and subjected to FACS analysis (n = 3–9 per group). For the identification of infiltrated neutrophil populations, cells were stained with fluorophore-conjugated different extracellular marker proteins: CD45, CD11b or Ly6G. (a) Representative FACS images are shown. (b,c) Bar graph represents (b) the ratio of the neutrophil population to the total CD45⁺ subset (%) and (c) the absolute numbers of neutrophils. (d,e) Immunohistochemistry and neutrophil count of liver samples 24 h after challenge with 20 mg/kg of ConA (n = 3–8 per group). Black arrows indicate neutrophils. Scale bar, 100 μm. MPF: medium power field. Data are means ± SEMs. *P < 0.05 (Tukey-Kramer HSD test after the one-way ANOVA).