Figure 5 | Scientific Reports

Figure 5

From: Small heterodimer partner negatively regulates C-X-C motif chemokine ligand 2 in hepatocytes during liver inflammation

Figure 5

SHP deficiency in liver affects neutrophil migration in vitro. (a) In vitro neutrophil migration to the liver was determined using the TX ChemoTx System. Liver extracts were obtained from WT or Shp KO mice 1 h after either PBS or 15 mg/kg ConA-treatment (n = 3 per group), and neutrophils were freshly isolated from bone marrow of WT mice. Isolated neutrophils pre-labeled with CellTracker Green were placed in the upper chamber and liver extract was added to the lower chamber. (b,d) Representative images of migrated neutrophils are shown. (b) Fluorescence image and (d) bright field image. Scale bar, 100 μm. (c,e) Fluorescence density reads from the lower chamber were used to quantify neutrophil migration. RFU: relative fluorescence units. Data are means ± SEMs of at least 3 individual experiments. *P < 0.05 (Tukey-Kramer HSD test after the one-way ANOVA).

Back to article page