Figure 1

(A–C) Recorded currents at 0 mV potential by applying an increased negative pressure from 0 to −80 mmHg on HEK cells transfected with kcnk2a-pIRES-2-eGFP in CA configuration (n = 3) (A) on HEK cells transfected with pIRES-2-eGFP in CA (n = 4) (B) and their corresponding current/pressure curves (C). (D–F) Recorded currents at 0 mV potential by applying an increased negative pressure from 0 to −80 mmHg on HEK cells transfected with kcnk2a-pIRES-2-eGFP in IO configuration (n = 7) (D) on HEK cells transfected with pIRES-2-eGFP in IO configuration (n = 4) (E) and their corresponding current/pressure curves (F). ^*P value < 0.05; ^**P value < 0.01. (G) Recorded currents in IO configuration by applying a voltage ramp going from −100 to +100 mV on HEK cells transfected with kcnk2a-pIRES-2-eGFP at pH 7.2 (black line) at pH 6.2 (red line) at pH 6.2 (blue line) (n = 9). Recorded currents on HEK cells transfected with pIRES-2eGFP at pH 5.2 (green line) (n = 3). (H) Typical graph showing the current amplitudes at 0 mV potential for each test conditions, ^*P value < 0.05; ^**P value < 0.01.