Figure 5

Recorded currents in IO configuration by applying an increasing negative pressure protocol from 0 to −80 mmHg by – 10 mmHG pressure step increments on HEK cells transfected with kcnk2a-pIRES-2-eGFP (control condition) (n = 7) (A) in the presence of 10 µM of spadin into the pipette medium (n = 6) (B) and their corresponding current/pressure curves (C). Recorded currents in IO configuration by applying an increasing negative pressure protocol from 0 to −80 mmHg by – 10 mmHG pressure step increments on HEK cells transfected with kcnk2b-pIRES-2-eGFP (control condition) (n = 7) (D) in the presence of 10 µM of spadin into the pipette medium (n = 6) (E) and their corresponding current/pressure curves (F). ^*P value < 0.05, ^***P value < 0.001.