Figure 6

Immuno-electron microcopy staining for La protein in exosomes. Exosomes were fixed overnight in 4% paraformaldehyde and immunostained for La using 10 nm gold labelled secondary antibody. La staining was observed in exosomes isolated from all three cell lines (B95.8, BL30-B95.8, BL30). Immunostaining for EBNA1 was included as a negative control, hence no signals was observed in any of the exosome preparations (c,f and e). Scale bar = 100 nm.