Figure 5
From: Expression of the Hutchinson-Gilford Progeria Mutation Leads to Aberrant Dentin Formation
Paracrine factors induced by progerin stimulate odontogenic differentiation of dental pulp cells. (a) The transcript levels of genes for soluble factors were analyzed by real-time qPCR of MDPC-23 transfected with Δ50 lamin A, WT lamin A and the negative control. (b,c) Mineralization ability of dental pulp cells treated with conditioned media from MDPC-23 cells of Δ50 lamin A (Δ50-CM), WT lamin A (WT-CM) and the negative control (Control-CM) was exhibited by alizarin red staining (b) and evaluated (c). (d) The transcript levels of odontogenesis-related genes were analyzed by real-time qPCR with dental pulp cells treated with conditioned media of Δ50 lamin A (Δ50-CM), WT lamin A (WT-CM) and the negative control (Control-CM) for 2 days. Significance was assigned for p-values as indicated. Bars, 40 μm (b).
