Figure 6
From: Expression of the Hutchinson-Gilford Progeria Mutation Leads to Aberrant Dentin Formation
Ctgf induced by progerin stimulates odontogenic differentiation of dental pulp cells. (a) The expression of Ctgf in odontoblasts was analyzed with whole cell extract from MDPC-23 transfected with Δ50 lamin A, WT lamin A and the negative control by Western blot analysis using an antibody for Ctgf. The samples shown were derived from the same experiment, and all gels/blots were processed under the same experimental conditions. β-actin was used as a loading control. Cropped images are displayed here; the original full-size blots are provided in Supplementary Fig. S7. (b) Reduced mineralization of mouse dental pulp cells by neutralization of Ctgf using polyclonal anti-Ctgf IgG. Alizarin red staining was performed with mouse dental pulp cells treated with conditioned media from the culture of MDPC-23 cells expressing Δ50 lamin A (Δ50-CM) and WT lamin A (WT-CM) supplemented with polyclonal anti-Ctgf IgG (2 μg/ml) and control IgG for 2 days. (c,d) Mineralization ability of dental pulp cells treated with different amounts of recombinant Ctgf peptide was exhibited by alizarin red staining (c) and evaluated (d). Significance was assigned for p-values as indicated. Bars, 40 μm (c).
