Figure 4
From: Functions of CsGPA1 on the hypocotyl elongation and root growth of cucumbers
Immunohistochemical analysis of CsGPA1 in cucumber leaves, root tips, and stems. Anti-CsGPA1 monoclonal antibody was used to analyze transverse sections of the root tips of 4-leaf-old plants (a–c). Control section treated with pre-immune serum revealing the absence of immunoreactivity in the root tip (b). Longitudinal sections of the petioles of 15-d-old plants (d,e), and stem cross sections of 30-d-old plants (f,g). (c) Magnified view of images in (a). (e,g) Magnified view of the images in the boxes of (d and f). Scale = 20, 50, 100, and 200 µm. Black arrows, nucleus; blue arrows, cell layers of root meristem; black arrowheads indicate the specific distributions of immunostaining in the root tip cortex, epidermal, endodermal, parenchymal, phloem cells, sieve elements, and companion cells. SE, sieve element; CC, companion cells; X, xylem; EP, external phloem; IP, internal phloem.
