Figure 5

Seed germination in transgenic cucumber lines. (a) Images of germinated seeds 0 and 3 d after germination from transgenic cucumber lines. Scale = 1 cm. (b) Seed germination rate of transgenic cucumber lines after 24 h and 48 h of darkness. (c) Relative CsGPA1 expression and western blot with anti-CsGPA1 monoclonal antibody at 6 days after germination in different transgenic cucumber lines. (d) Weight (g) per thousand seeds of different transgenic cucumber lines. (e) Seed length of transgenic cucumber lines. (f) Seed width of transgenic cucumber lines. Seeds were considered to have germinated if the emerging radicle grew to 3 mm. Seed germination rates (%) were analyzed at the indicated time points. Data are presented as the mean ± standard deviation of three independent replicates with at least 20 seeds counted per replicate. Values that differed significantly from the WT values are indicated. *p < 0.05 according to a Bonferroni post-hoc test. OE, CsGPA1-overexpressing transgenic plants; RNAi, CsGPA1-silenced transgenic plants.