Figure 6
From: Functions of CsGPA1 on the hypocotyl elongation and root growth of cucumbers
Changes to the morphological characteristics of transgenic cucumber lines. (a,b) Morphology of 3-d-old (a) and 6-d-old (b) WT and transgenic seedlings grown in darkness. WT and transgenic seedlings were grown in 9 cm diameter Petri dishes containing two layers of filter paper moistened with 5 mL of sterilized distilled water. Plates were incubated for 6 d at 30 °C in darkness. Seeds were considered to have germinated if the emerging radicle grew to 3 mm. WT (middle), OE (CsGPA1-overexpressing transgenic plants, at left), and RNAi (CsGPA1-silenced transgenic plants, at right) seedlings. Scale = 1 cm. Hypocotyl (c) length and (e and j) parameters, (d,k) cotyledon parameters, (g–i and l) root parameters, and (f) stem diameter of 6-d-old seedlings. The WT, OE, and RNAi seedlings were grown on plates supplemented with 5 mL of deionized water and then measured. Error bars correspond to the standard error of the mean. Values and data are presented as the mean ± standard deviation of three independent replicates with at least 20 seedlings counted per replicate. Values that differed significantly from the WT values are indicated. *p < 0.05 according to a Bonferroni post-hoc test.
