Figure 4

SLC3A2/LAT1 complex is upregulated by HCV infection. (A) Huh7.5 cells were either mock-infected or infected with Jc1 for the indicated time intervals. Both mRNA (top panel) and protein (bottom panel) levels of LAT1 were analyzed either by qRT-PCR or immunoblot analysis, respectively. (B) Huh7.5 cells were treated with either reducing or non-reducing buffer as described in Methods. Total cell lysates were subjected to native gel electrophoresis and immunoblotted with either SLC3A2 or LAT1 antibodies, respectively. (C) Huh7.5 cells were either mock-infected or infected with Jc1 for 4 and 8 days, respectively. Total cell lysates prepared by native lysis buffer were immunoblotted with the indicated antibodies.