Figure 7

SLC3A2 is not involved in the replication, translation, and assembly/release steps of the HCV life cycle. (A) Huh7 cells harboring HCV subgenomic replicon derived from genotype 1b were transfected with the indicated siRNAs. At 72 h after siRNA transfection, both RNA (left) and protein (right) levels were analyzed by qRT-PCR and immunoblot assay, respectively. (B) Huh7.5 cells were transfected with the indicated siRNAs. At 48 h after siRNA transfection, cells were further transfected with pRL-HL dual luciferase and a pCH110 β-galactosidase plasmid. At 48 h after transfection, relative luciferase activity was determined. (C) (Top panel) A schematic illustration of the experimental design. (Middle panels) Huh7.5 cells were infected with Jc1 for 48 h and then transfected with 40 nM of the indicated siRNAs. At 48 h after transfection, both intracellular RNA (left) and protein (right) levels were determined by qRT-PCR and immunoblot assay, respectively. (Bottom panels) Naïve Huh7.5 cells were infected with Jc1 in supernatant harvested from the first infection. At 48 h postinfection, both RNA (left) and protein (right) levels were determined by qRT-PCR and immunoblot assay, respectively.