Figure 8
From: Inhibition of TBK1/IKKε Promotes Regeneration of Pancreatic β-cells
PIAA improves glucose control and β-cell mass in the STZ-induced diabetic mouse model. (A–H) STZ-induced diabetic mice were treated with vehicle or PIAA for 2–3 weeks after reaching > 300 mg/dL fed glucose values (n = 6–8 mice per group). (A) PIAA caused reduction of hyperglycemia (non-fasting glucose measurement) relative to vehicle-treated animals. PIAA-treated animals showed improved (B) glucose and (C) insulin tolerance. Confocal images of diabetic pancreata treated with (D) vehicle and (E) PIAA, respectively, stained for Ki67 (red, white arrows), Insulin (green), and Glucagon (blue). (F) The percentage (mean ± SD) of Ki67 and Insulin-double positive cells in diabetic islets increased with PIAA treatment (0.7 ± 1.0% (vehicle) and 3.1 ± 1.2% (PIAA)). Quantification (mean ± SD) of (G) β-cell area (fold change, 1.0 ± 0.3 (vehicle) and 1.9 ± 0.1 (PIAA)) and (H) insulin content (51.8 ± 1.9 μg/g (vehicle) and 114.0 ± 37.7 μg/g (PIAA)) in diabetic pancreata treated with vehicle or PIAA. *P < 0.05; **P < 0.01; ***P < 0.001.
