Figure 2 | Scientific Reports

Figure 2

From: Nanogel tectonic porous 3D scaffold for direct reprogramming fibroblasts into osteoblasts and bone regeneration

Figure 2

Osteoblasts efficiently adhered to and proliferated on the fibronectin-coated NanoCliP-FD gel. (a) Preparation of NanoCliP-FD gel with dOBs. (b,c) XOL-transduced cells were seeded into rhodamine-labeled NanoCliP-FD gel that had been coated with either RGDC or fibronectin. After culturing for the indicated days, the samples were stained with calcein-AM (b) or phalloidin/Hoechst 33342 (c), and CLSM images at magnifications of objective lenses of x2.5 (upper) and x20 (lower) are shown. (d) XOL-transduced cells were seeded into non-coated (−), RGDC-conjugated, and fibronectin-coated NanoCliP-FD gel. After culturing for the indicated days, cell viability was evaluated by tetrazolium-based assay. Values are means ± SD. N = 3. **p < 0.01 vs. day 1.

Back to article page