Figure 1
R430 is effective against acyclovir-resistant strains of HSV-1. Vero cells were infected with ACV-resistant HSV-1 strains Tk- and PAAv, or HSV-1 KOS strain, and incubated with acyclovir or R430 at concentrations varying from 0.1–50 µM. At 48 hours post-infection cells were fixed and stained for ICP4 protein, counterstained with Hoechst 33342, and imaged with Nikon AS1 microscope. Cells were counted and IC50 was determined using the drc package for R, based on the proportion of positive-staining cells. CC50 for R430 was determined using drc package based on total number of remaining cells. No CC50 is reported for ACV due to negligible toxicity.
