Table 1 Effects of R430 on HSV-1 and host gene transcription.

From: R430: A potent inhibitor of DNA and RNA viruses

Assay conditions

RIN

Host genome reads

Viral genome reads

Uninfected, no R430

9.43 (0.15)

103.89 × 106 (2.33 × 106)

Not detected

Uninfected, with R430

9.53 (0.06)

100.46 × 106 (8.65 × 106)

Not detected

Infected, without R430

9.60 (0.17)

93.99 × 106 (8.25 × 106)

21,571 (2505.59)

Infected, with R430

9.5 (0.10)

105.82 × 106 (13.39 × 106)

1,079 (223.95)

  1. hiPSC-neurons were incubated with HSV-1 (MOI 0.3) and R430 (10 µM) in triplicate; appropriate control conditions were included. Total RNA was extracted, quantified and sequenced following ribosome depletion. Sequences were trimmed and mapped to GRCH38 using Ensembl (release 82) to estimate human transcript counts. The remaining unmapped reads were collected and mapped to the Human herpesvirus 1 strain KOS genome (GenBank: JQ780693.1) to estimate viral transcript counts. The mean number of human and viral transcripts are shown (standard deviations in brackets). The following comparisons were statistically significant:
  2. Viral transcripts in (uninfected + R430) vs (uninfected + vehicle) conditions: t = −14.11, p = 0.00015; Human transcripts in (uninfected + R430) vs (uninfected + vehicle): t = −0.61, p = 0.573.
  3. Human transcripts in (infected + R430) vs (infected without R430): t = 1.27, p = 0.271.
  4. There were no significant changes in human transcripts in the HSV-1 infected vs uninfected conditions (t = 0.11, p = 0.13). RIN: RNA integrity number.
  5. RIN: RNA integrity number. All values shown as mean (standard deviation) from triplicate experiments. R430 (10 µM) was used where specified.
  6. Viral expression in (uninfected + R430) + vs uninfected: t = −14.11, p = 0.00015.
  7. Effects of R430 on host: (uninfected + R430) vs uninfected: t = −0.61, p = 0.573.
  8. (infected + R430) vs (infected without R430): t = 1.27, p = 0.271.
  9. Effects of virus on host: infected vs uninfected: t = 0.11, p = 0.13.
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