Figure 3

Overexpression of miR-133a in airway epithelial cells induces EMT. (a,b) Beas-2b cells were mock transfected or transfected with control or miR-133a mimics and cultured in full growth medium for 5 days. Cells were imaged with an inverted microscope (a) and then harvested for western blot analysis of indicated proteins (b). (c,d) NHBE cells were mock transfected or transfected with control or miR-133a mimics and cultured in full growth medium for 3 days before imaging and western blot analysis of indicated proteins. Experiments were conducted at least three times, and representative results are shown. Each group of blots in (b) and (d) was cropped from different parts of the same gel. Unprocessed original scans of the blots are shown in Supplementary Fig. S5. Data are mean ± S.E. with *P < 0.05. Scale bars, 100 µm.