Figure 3
From: The Cell Activation Phenomena in the Cold Atmospheric Plasma Cancer Treatment
The research strategy to demonstrate the existence of the activation state. The activation state of the cancer cells was achieved by a direct CAP jet treatment. A quick (<20 s after the CAP treatment) removal of the medium after the CAP treatment was used to eliminate the effect of long-lived reactive species in the medium. The quick removal of the medium sustained the cell’s growth and retained the activation state of cancer cells. The comparison between the cytotoxicity of the reactive species-based treatment such as a H2O2 treatment on the cancer cells activated by CAP jet and that on the cancer cells without the CAP activation revealed the existence of the activation state. The protocols in each case was the same throughout the whole manuscript. Case 1, Case 2, Case 3, and Case 4 responded to ‘One step CAP treatment’, ‘Activated cells cultured in DMEM without reactive species’, ‘H2O2 treatment on the cells with activation’, and ‘H2O2 treatment on the cells without activation’, respectively.
