Figure 5
From: The Cell Activation Phenomena in the Cold Atmospheric Plasma Cancer Treatment
CAP can activate the cancer cells into a sensitized state, in which the cells are very sensitive to the reactive species. (a) The cytotoxicity of NO2− (50 μM) and NO3− (50 μM) on the activated cancer cells. (b) The cytotoxicity of PSM on the activated cancer cells. The basic research strategy was the same as that illustrated in Fig. 3. The protocols to prepare the NO2-DMEM and NO3-DMEM were illustrated in Materials and Methods. PSM was made by treating DMEM (1 mL/well) in 12-well plate by the CAP jet following the method in Materials and Methods. PSM was quickly moved to affect cancer cells’ growth. The experiments in triplicate was dependently repeated for three times. The results are shown as the mean ± sd. Student’s t-tests were performed and the significance was indicated as ***p < 0.005, **p < 0.01, *p < 0.05, respectively.
