Figure 3
From: SB772077B, A New Rho Kinase Inhibitor Enhances Aqueous Humour Outflow Facility in Human Eyes
Effect of SB77 on HTM cell viability by MTT assay. (A) HTM cells treated with SB77 showed more than 85% (Mean percentage viability: 87.8 ± 5.9) cell viability at the studied concentrations (0.1–100 µM). The values are represented as mean ± SD. Experiments were conducted in triplicate. (B) Terminal deoxyribonucleotidyl transferase (TdT) - mediated fluroscein-16-dUTP nick end-labelling (TUNEL) staining on HTM cells after treatment with DNAse I (i), vehicle control (ii), SB77 (iii) and Y27632 (iv) for 24 h. TUNEL positive cells (green) were detected only in HTM cells treated with DNase I (i) (Positive control). No TUNEL positive cells were detected in other treatment groups. Cell nuclei were stained with DAPI (blue). A detailed protocol for MTT and TUNEL assay was given in method section.
