Figure 4
From: SB772077B, A New Rho Kinase Inhibitor Enhances Aqueous Humour Outflow Facility in Human Eyes
Effect of SB77 on TM actin cytoskeleton, focal adhesion (vimentin) and ECM (fibronectin). (A) Treatment with SB77 caused decreased positive staining of stress fibers, vimentin, vinculin, fibronectin and collagen 1 A staining. HTM cells treated with Y27632 showed similar findings; however, the observed effect was more prominent in SB77-treated cells as compared to Y27632-treated cells. The images are representative of three independent experiments. Green fluorescence indicates positivity for F-actin, vimentin, vinculin, fibronectin and collagen 1 A. Cell nuclei were stained with DAPI (blue). (B) Immunoblot analysis showing the effect of SB77 on the expression of focal adhesion and ECM protein. Decreased expression of vinculin, vimentin and ECM proteins was observed in HTM cells treated with both inhibitors. Treatment with SB77 showed a marked decrease as compared to vehicle control and Y27632-treated cells. The cropped images are used in the figure, and full length blots for the same are presented in S. Fig. 3. (C) Densitometry analysis of the results of 4B. Experiments were conducted in triplicate. Data are expressed as mean ± SD. *p < 0.05; **p < 0.001; ***p < 0.0001, paired Student’s t-test.
