Figure 1

Effect of white-nose syndrome on level of Myotis lucifugus coronavirus (Myl-CoV) RNA in hibernating little brown bats (M. lucifugus). Relative transcript levels for the coronavirus RNA polymerase gene for each bat are depicted as reciprocal of Cycle threshold (Ct) normalized separately (ΔCt) for levels of glyceraldehyde 3-phosphate dehydrogenase (GAPDH) transcripts in each sample. The horizontal bar represents the mean while the vertical bar indicates standard deviation from the mean. Significance (p value) is as calculated with an independent Mann-Whitney test. Virus-infected bats had lower 1/ΔCt values for coronavirus RNA than co-infected bats. The average fold-differences between virus-infected and co-infected bats were calculated from the difference between the average ΔCt values.