Figure 3
From: Inhibiting ex-vivo Th17 responses in Ankylosing Spondylitis by targeting Janus kinases
JAK inhibitors inhibit multiple cytokine-driven STAT phosphorylation events. Inhibition of STAT phosphorylation by JAK inhibitors (Tofa, JAK3 > JAK1/2; Ruxo, JAK2 > JAK1; Bari, JAK1/2 > TYK2; CEP, JAK2) in freshly isolated AS PBMC (n = 5–6) upon cytokine stimulation assessed by intracellular Flow Cytometry. Stimulation with (a) IL-6, (b) IFNα and (c) IL-7 gated on CD4+ T cells and with (d) GM-CSF gated on CD14+ Monocytes. Panel on the left shows exemplary flow cytometry plot (light grey filled curves in each panel show unstimulated control staining), middle panel shows fold increase of mean fluorescence intensity (MFI) compared to unstimulated control and right panel shows frequency of phosphorylated STAT of parental population. Statistical analysis: mean ± SEM, 2-way ANOVA followed by Bonferroni’s method for multiple comparisons.
