Figure 3

Gene expression in the skin, and Ab production in Il17c^−/− mice. (a) Relative mRNA expression levels of cytokines and chemokines in the ear skin of wild-type and Il17c^−/− mice 24 hours after FITC challenge. V = vehicle treatment. Wild-type mice (vehicle, n = 3; FITC, n = 5) and Il17c^−/− mice (vehicle, n = 3; FITC, n = 6). (b) Relative mRNA expression levels of cytokines and chemokines in the ear skin of wild-type and Il17c^−/− mice 24 hours after DNFB challenge. V = vehicle treatment. Wild-type mice (vehicle, n = 3; FITC, n = 5) and Il17c^−/− mice (vehicle, n = 3; FITC, n = 5). (c) Sera were collected from mice 72 hours after FITC challenge. The levels of FITC-specific Ig subsets in sera were determined by ELISA. N = naïve. Wild-type mice (naive, n = 8; FITC, n = 30) and Il17c^−/− mice (naive, n = 4; FITC, n = 32). (d) Sera were collected from mice 72 hours after DNFB challenge. The levels of DNP-specific Ig subsets in sera were determined by ELISA. N = naïve. Wild-type mice (naive, n = 8; DNFB, n = 27) and Il17c^−/− mice (naive, n = 5; DNFB, n = 30). The data show the mean + SEM. *p < 0.05, **p < 0.01 and ***p < 0.001: vehicle vs FITC or DNFB; and ^†p < 0.05 and ^††p < 0.01: wild-type mice vs. Il17c^−/− mice. Analyses by Mann-Whitney U-test, two-tailed (a, b) and Student’s t-test, two-tailed (c, d). NS = not significant.